主要功能
●對自然水體中的藍(lán)藻、綠藻、硅/甲藻和隱藻自動分類（定性）
●自動測量水樣中藍(lán)藻、綠藻、硅/甲藻和隱藻的葉綠素 a 含量（定量）和總?cè)~綠素 a 含量
●一杯自然水樣，同時(shí)獲得藍(lán)藻、綠藻、硅/甲藻和隱藻的光合活性：
●光合效率和光合速率（相對電子傳遞速率）
●快速光曲線并進(jìn)行擬合
●藻類的潛在最大光合效率
●藻類的光保護(hù)能力
●藻類耐受強(qiáng)光的能力
●用戶可做自己的參考光譜
測量參數(shù)
Fo, Fm, F, Fm', Fv/Fm, Y(II) 即 ΔF/Fm', ETR, a, Ik, Pm, PAR 和葉綠素含量等
應(yīng)用領(lǐng)域
●主要用于水生生物學(xué)、水域生態(tài)學(xué)、海洋學(xué)、湖沼學(xué)、水質(zhì)預(yù)警、微藻生理學(xué)、微藻抗逆性等領(lǐng)域，對于了解自然水樣中藻類種群的動態(tài)變化、水華預(yù)警、野外水體中光合作用的時(shí)空變化、校正初級生產(chǎn)力的計(jì)算等有較大幫助。
●特別適于浮游植物動力學(xué)研究和有害水華的早期預(yù)警。
主要技術(shù)參數(shù)
●主控單元：金屬外殼，包含所有的光電元件及樣品測量室
●測量光： LED，440 nm，480 nm，540 nm，590 nm 和 625 nm，5 波長脈沖調(diào)制測量光，2 檔強(qiáng)度設(shè)置，8 檔調(diào)制頻率設(shè)置， 3 檔測量光自動高頻設(shè)置
●光化光：板載多波長 LED 陣列，440 nm，480 nm，540 nm，590 nm，625 nm 和 420-640 nm（白光），提供持續(xù)光化光，最高可達(dá) 1400 μmol m-2 s-1，快速動力學(xué)閃光高達(dá) 7000 μmol m-2 s-1，飽和脈沖最高可達(dá) 5000 μmol m-2 s-1
●遠(yuǎn)紅光：725 nm LED
●信號檢測：基于 H-10720 光電傳感器模塊的光電倍增檢測器
●標(biāo)準(zhǔn)檢測過濾器：> 650 nm 長通濾光片
●高分辨率的光電倍增管，葉綠素濃度檢測限低至 0.1μg/L，適用野外采集的藻濃度很稀的樣品
升級的技術(shù)特點(diǎn)
●可提供 5 種波長的脈沖調(diào)制測量光和光化光
●板載 LED 陣列芯片技術(shù)
●可實(shí)時(shí)進(jìn)行四種藻的分類
●可進(jìn)行標(biāo)準(zhǔn) PAM 測量及不同波長強(qiáng)光化光誘導(dǎo)的毫秒級熒光上升動力學(xué)分析
●可測定光系統(tǒng) II 功能性捕光截面積
●內(nèi)置自動測量程序，易于操作
PHYTO-PAM-II vs PHYTO-PAM
便攜式 PHYTO-PAM-II | PHYTO-PAM |
5 種不同波長的測量光用于生物體內(nèi)不同類型的天線色素?zé)晒獾募ぐl(fā) | 4 種不同波長的光 |
4 種色素類型的在線分類 | 3 種色素類型的在線分類 |
6 種波長的光化光 | 1 種波長的光化光 |
可以分別測量綠藻，藍(lán)藻，硅/甲藻以及含有藻紅蛋白的有機(jī)體，如隱藻不同波長下 PSII 的活性 | 無該功能 |
緊湊型設(shè)計(jì) | 需要組裝 |
增加了快速動力學(xué)操作模式，可通過強(qiáng)光化光脈沖，測量不同波長的 O- I1 熒光上升動力學(xué)曲線 | 無該功能 |
通過測定不同光質(zhì)和光合生物色素復(fù)合體，獲得光系統(tǒng) II 功能性捕光截面積的信息即 σPSII | 無該功能 |
通過 FluoRed 熒光標(biāo)準(zhǔn)將參考光譜校準(zhǔn)標(biāo)準(zhǔn)化 | 無該功能 |
獲得的參考光譜可在不同設(shè)備及用戶間互換使用 | 參考光譜不能互換 |
選購指南
一、實(shí)驗(yàn)室測量基本款
系統(tǒng)組成：實(shí)驗(yàn)室版主機(jī)，激發(fā)檢測單元，懸浮液的光學(xué)單元，球形光量子傳感器，工作臺，軟件等
注意：新版 PHYTO-PAM-II 必須配置光量子傳感器，校準(zhǔn)光強(qiáng)后參考光譜可在不同儀器間通用
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實(shí)驗(yàn)室測量基本款 |
二、野外便攜緊湊款
系統(tǒng)組成：緊湊型主機(jī)，球形光量子傳感器，軟件等
注意：新版 PHYTO-PAM-II 必須配置光量子傳感器，校準(zhǔn)光強(qiáng)后參考光譜可在不同儀器間通用
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便攜式緊湊款 |
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五種測量光通道 | 區(qū)分四種藻類 | 通用型參考光譜 |
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測量慢速動力學(xué)曲線 淬滅分析，暗弛豫分析 | 測量快速光曲線 光響應(yīng)曲線 | 測量快速動力學(xué)曲線 分析PSII功能性捕光截面 |
三、其他可選附件
1，WATER-S：攪拌器（推薦選配），利用內(nèi)置電池驅(qū)動的馬達(dá)帶動攪拌棒旋轉(zhuǎn)，對樣品杯里的懸浮液進(jìn)行攪拌。需配置攪拌棒 WATER-R。
2，WATER-R：攪拌棒（標(biāo)配10根）
產(chǎn)地：德國WALZ
參考文獻(xiàn)
數(shù)據(jù)來源：光合作用文獻(xiàn) Endnote 數(shù)據(jù)庫，更新至 2021 年 1 月，文獻(xiàn)數(shù)量超過 10000 篇
原始數(shù)據(jù)來源：Google Scholar
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