主要功能
單獨(dú)或同步測量葉綠素?zé)晒夂?P700
兩個(gè)光系統(tǒng)的誘導(dǎo)動(dòng)力學(xué)曲線(包括快相和慢相)
兩個(gè)光系統(tǒng)的快速光曲線和光響應(yīng)曲線
淬滅分析、暗馳豫分析
典型的 P700 曲線測量
通過葉綠素?zé)晒夂?nbsp; P700 的同步測量獲知兩個(gè)光系統(tǒng)的電子傳遞動(dòng)力學(xué)、電子載體庫的大小、圍繞 PSI 的環(huán)式電子傳遞動(dòng)力學(xué)等
通過測量 P515/535 信號(hào)變化測量跨膜質(zhì)子動(dòng)力勢 pmf 及其組分跨膜質(zhì)子梯度 ΔpH 和跨膜電位 Δψ
“P515 Flux”信號(hào)能原位反映活體樣品處于穩(wěn)態(tài)的偶聯(lián)電子和質(zhì)子的流動(dòng)速率
通過測量 NADPH 熒光估算 NADP 的還原程度
通過測量 9-AA 熒光來估算跨膜質(zhì)子梯度 ΔpH
測量參數(shù)
PS II參數(shù): Fo, Fm, F, Fm’, Fv/Fm, Y(II) 即 △F/Fm’, Fo’, qP, qL, qN, NPQ, Y(NPQ), Y(NO) 和 ETR(II) 等
PS I參數(shù): P700, Pm, Pm’, P700ox, Y(I), Y(ND), Y(NA) 和 ETR(I) 等
P515/535參數(shù):質(zhì)子動(dòng)力勢pmf,跨膜質(zhì)子梯度 ΔpH,跨膜電位 Δψ 等
NADPH/9-AA參數(shù):NADP 的還原程度,ΔpH 等
其他測量參數(shù):Post-Illumination(鼓包),PQ-Pool(PQ庫)等
應(yīng)用領(lǐng)域
相當(dāng)于兩臺(tái) PAM-101/ 102/ 103 的功能,可同時(shí)測量光系統(tǒng)II活性(調(diào)制葉綠素?zé)晒猓┖凸庀到y(tǒng)I活性(P700 吸收變化),可用于、植物生理學(xué)、農(nóng)學(xué)、林學(xué)、園藝學(xué)等領(lǐng)域光合作用機(jī)理研究。
擴(kuò)展模塊 P515/535 可測量跨膜質(zhì)子動(dòng)力勢 pmf 及其組分跨膜質(zhì)子梯度 ΔpH 和跨膜電位 Δψ 等,是葉綠素循環(huán)和光保護(hù)研究的強(qiáng)大工具。
擴(kuò)展模塊NADPH/9-AA,可測量 NADPH 熒光和 9-AA 熒光,估算 NADP 的還原程度和跨膜質(zhì)子梯度 ΔpH。
主要技術(shù)參數(shù)
主機(jī):通用型(DUAL-C),可接標(biāo)準(zhǔn)檢測器,可擴(kuò)展 P515/535,NADPH/9AA 等多種模塊。
P700 雙波長測量光:LED,830 nm 和 875 nm
PSII 熒光測量光:LED,460 nm(DUAL-DB)或 620 nm(DUAL-DR)
紅色光化光:LED 陣列,635 nm;最大連續(xù)光強(qiáng) 3000 μmol m-2 s-1
藍(lán)色光化光:LED,460 nm;最大連續(xù)光強(qiáng) 1100 μmol m-2 s-1
單周轉(zhuǎn)飽和閃光(ST):200000 μmol m-2 s-1,5~50 μs 可調(diào)
多周轉(zhuǎn)飽和閃光(MT):20000 μmol m-2 s-1,1~1000 ms 可調(diào)
遠(yuǎn)紅光:720nm
選購指南
一、高等植物葉片測量基本款
系統(tǒng)組成:通用型主機(jī),標(biāo)準(zhǔn)版檢測單元,數(shù)據(jù)線,工作臺(tái),軟件等
注意:高等植物葉片測量紅光檢測器(Dual-DR)和藍(lán)光檢測器(Dual-DB)可任選其一
高等植物葉片測量基本款 |
二、懸浮樣品測量基本款
系統(tǒng)組成:通用型主機(jī),標(biāo)準(zhǔn)版檢測單元,懸浮液的光學(xué)單元,數(shù)據(jù)線,工作臺(tái),軟件等
注意:藻類測量時(shí),藍(lán)藻請選擇紅光檢測器(Dual-DR),其他藻類可選藍(lán)光檢測器(Dual-DB)
懸浮樣品測量基本款 |
同步測量 PSII(紅色)和 PSI(藍(lán)色)的誘導(dǎo)曲線 | 同步測量 PSII(紅色)和 PSI(藍(lán)色)的光響應(yīng)曲線 | 典型的 P700 測量曲線 |
| | |
打開飽和脈沖時(shí)葉綠素?zé)晒庑盘?hào)(紅色)和 P700(藍(lán)色)信號(hào)變化 | 以線性時(shí)間測量的熒光快速動(dòng)力學(xué)曲線 | 以對數(shù)時(shí)間測量的熒光快速動(dòng)力學(xué)曲線 |
三、其他擴(kuò)展模塊
擴(kuò)展測量一:P515/535模塊
P515/535 模塊是 WALZ 公司為 DUAL-PAM-100 設(shè)計(jì)的測量模塊,可以直接連接 DUAL-PAM-100 的主機(jī),測量 550-510 nm 的差式吸收以及 535 nm 波長的信號(hào)變化。P515/535 模塊可以測量光合器官的跨膜質(zhì)子動(dòng)力勢(pmf)、跨膜電位(Δψ)、跨膜質(zhì)子梯度(ΔpH)和玉米黃素(Zea)變化等內(nèi)容。此外,該模塊還提供一種特殊的 “P515 Flux” 操作模式,可讓光化光以光-暗脈沖形式打開-關(guān)閉(1/1調(diào)制光/暗),原位測量活體樣品處于穩(wěn)態(tài)的偶聯(lián)電子和質(zhì)子的流動(dòng)速率。 | |||
通過測量 P515 變化得出質(zhì)子動(dòng)力勢(pmf)兩個(gè)組分 Δψ 和 ΔpH | 通過測量535 nm變化得出質(zhì)子動(dòng)力勢(pmf)及其組分ΔpH | 同步測量P515和535 nm信號(hào)的光響應(yīng)曲線 |
擴(kuò)展測量二:NADPH/9-AA 模塊
NADPH/9-AA 模塊是 WALZ 公司為 DUAL-PAM-100 設(shè)計(jì)的測量模塊,可以直接連接 DUAL-PAM-100 的主機(jī),測量 NADPH 熒光和 9-AA 熒光。NADPH 熒光可用于估算 NADP 的還原程度,9-AA 熒光用于估算跨膜質(zhì)子梯度 ΔpH。該模塊的一個(gè)很大特色是與標(biāo)準(zhǔn)探頭聯(lián)用,在國際上第一次做到了同步測量葉綠素?zé)晒馀c NADPH 熒光。
NADPH 探頭圖示 | 同步測量 NADPH 熒光(藍(lán)色)與葉綠素?zé)晒猓?span style="color: rgb(255, 0, 0);">紅色) |
擴(kuò)展測量三:與光合儀 GFS-3000 聯(lián)用
|
專為 DUAL-PAM-100 與 GFS-3000 的同步測量設(shè)計(jì),由特制葉室(帶溫度和PAR傳感器)、風(fēng)扇、導(dǎo)光桿、電子盒與支架構(gòu)成。同步測量時(shí),光源完全由 DUAL-PAM-100 的測量頭提供,氣體交換由 GFS-3000 的紅外分析器檢測,P700 和葉綠素?zé)晒庥?DUAL-PAM-100 的檢測器測量。 |
四、其他可選附件
1,Dual-DPD:單獨(dú)的光電二極管檢測器單元,通過導(dǎo)光桿連接到 ED?101US/MD 上,配置 NADPH 模塊時(shí)推薦選配。
2,Dual-DPM:單獨(dú)的光電倍增管檢測器單元,用于較稀的懸浮液熒光測量,需要裝配到 ED-101US/MD 上使用。必須要同時(shí)配置測量頭 DUAL-DB 或 DUAL-DR 來激發(fā)調(diào)制熒光。
3,ED-101US/T: 控溫裝置,安裝在 ED-101US/MD 上,為懸浮液控溫;可外接循環(huán)水浴來控溫,
4,US-SQS/WB: 球狀微型光量子探頭,可插入樣品杯中測量 PAR;由主機(jī) DUAL-C 控制。
5,PHYTO-MS:磁力攪拌器,連接到光學(xué)單元 ED-101US/MD 的底部對懸浮液進(jìn)行攪拌。
6,DUAL-OP:擬南芥等小葉片測量用適配器,特制透光小孔適配器套裝,直徑 7 mm、5 mm 和 3 mm,對于擬南芥等小葉片的 P700 測量非常重要!
產(chǎn)地:德國 WALZ
參考文獻(xiàn)
數(shù)據(jù)來源:光合作用文獻(xiàn) Endnote 數(shù)據(jù)庫,更新至 2021 年 1月,文獻(xiàn)數(shù)量超過 10000 篇
原始數(shù)據(jù)來源:Google Scholar
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