功能強(qiáng)大、操作簡(jiǎn)便、文獻(xiàn)眾多的葉綠素?zé)晒獬上裣到y(tǒng)
突變株快速篩選的強(qiáng)大工具
? 標(biāo)準(zhǔn)藍(lán)光版，450 nm，測(cè)葉片和真核藻類(lèi) ? 紅光版，620 nm，測(cè)藍(lán)藻和真核藻類(lèi) |
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主要功能
? 成像功能：對(duì)Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等參數(shù)進(jìn)行成像分析。測(cè)定調(diào)節(jié)性能量耗散Y(NPQ)，反映植物光保護(hù)能力，測(cè)定非調(diào)節(jié)性能量耗散Y(NO)，反映植物光損傷程度。
? 程序測(cè)量功能：可程序測(cè)量熒光誘導(dǎo)曲線、快速光曲線和暗弛豫，也可手動(dòng)測(cè)量；在測(cè)量過(guò)程中能自動(dòng)分析所有熒光參數(shù)的變化趨勢(shì)
? AOI功能：可在測(cè)量前或測(cè)量后任意選擇感興趣的區(qū)域（AOI），程序?qū)⒆詣?dòng)對(duì)選擇的AOI的數(shù)據(jù)進(jìn)行變化趨勢(shì)分析，并在報(bào)告文件中顯示相關(guān)AOI的數(shù)據(jù)。所有報(bào)告文件中顯示的數(shù)據(jù)都可導(dǎo)出到EXCEL文件中。
? 成像異質(zhì)性分析功能：對(duì)任意參數(shù)任意時(shí)間的成像，可在圖像上任意選取兩點(diǎn)，軟件自動(dòng)對(duì)兩點(diǎn)間的數(shù)據(jù)進(jìn)行橫向異質(zhì)性分析，并可導(dǎo)出到EXCEL文件中。
? 成像數(shù)據(jù)范圍分析功能：對(duì)任意參數(shù)任意時(shí)間的成像，可分析任意兩個(gè)熒光數(shù)值之間有多少個(gè)像素點(diǎn)，多少面積（cm2）。
? 突變株篩選功能：可跟據(jù)成像結(jié)果快速篩選光合、產(chǎn)氫/油、抗逆（抗鹽、抗旱、抗病等）等突變株。
? 微藻毒理研究功能：可同時(shí)測(cè)量96個(gè)微藻樣品（對(duì)照和處理組）的光合活性，軟件自動(dòng)給出處理組樣品相對(duì)于對(duì)照組的光合抑制百分比。
? 吸光系數(shù)測(cè)量功能：快速測(cè)量葉片的吸光系數(shù)。吸光系數(shù)測(cè)量光源： 16個(gè)紅光（650 nm）和16個(gè)近紅外（780 nm）LED，用于測(cè)量植物葉片或藻類(lèi)樣品PAR吸光系數(shù)。
? 監(jiān)測(cè)功能：可實(shí)時(shí)監(jiān)控箱內(nèi)環(huán)境狀態(tài)、機(jī)器狀態(tài)監(jiān)控區(qū)顯示目前程序及環(huán)境狀態(tài)
? 環(huán)境仿真功能：可程序化多組程序功能 (可多至6組)，可個(gè)別設(shè)定執(zhí)行天數(shù)，可設(shè)備單日環(huán)境參數(shù)（間隔可至分鐘），程序化環(huán)境參數(shù)
? 項(xiàng)目管理功能：預(yù)約存多次測(cè)量的植物影像及參數(shù)，延時(shí)（縮時(shí)）攝影功能
? 數(shù)據(jù)分析功能：可導(dǎo)出照片文件，可導(dǎo)出葉綠素?zé)晒鈪?shù)Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等
系統(tǒng)設(shè)計(jì)
? 可單獨(dú)作為葉綠素?zé)晒鈨x使用，方便帶到野外田間測(cè)量和成像；也可配置箱體式結(jié)構(gòu)，容量300L，能同時(shí)滿(mǎn)足植物培養(yǎng)和葉綠素?zé)晒獬上竦墓δ堋Ｏ潴w內(nèi)設(shè)有栽培區(qū)，可分2層、3層、4層等，調(diào)節(jié)高度進(jìn)行培養(yǎng)和測(cè)量。箱體內(nèi)有特殊消光圖層，避免反光造成的測(cè)量干擾。
? 系統(tǒng)配置觸摸式高清顯示屏（24寸），方便操作，支持1670萬(wàn)色彩和FHD高清畫(huà)質(zhì)。
? 系統(tǒng)處理器：I7處理器，16G緩存，512固態(tài)硬盤(pán)SSD，滿(mǎn)足快速數(shù)據(jù)處理和存儲(chǔ)要求。
? 供電：室內(nèi)使用220V，50Hz交流電，熒光儀單獨(dú)使用時(shí)，內(nèi)置可充電鋰電池供電，滿(mǎn)足4小時(shí)的野外測(cè)量
箱體環(huán)境控制（光照、溫度等）
? 溫度環(huán)控條件：15-40°C ±5°C（關(guān)燈）
? 光照系統(tǒng)：采用四合一LED光盤(pán)，白光5000k ±500k、紅光660nm±10nm、藍(lán)450nm±10nm、紅外光730nm±10nm，光盤(pán)面積29×40cm，四種光源可獨(dú)立自主控制，光質(zhì)強(qiáng)度可達(dá)PPFD ≧700μmols-1m-2@15cm，光照時(shí)間控制可程序化設(shè)定，設(shè)定時(shí)間可至1min，可仿真日出至日落光照變化。
? 控制系統(tǒng)：PID微電腦控制，可設(shè)定上晝夜循環(huán)程序
? 可選配二氧化碳和濕度控制單元
成像技術(shù)參數(shù)
Maxi探頭
? 熒光測(cè)量光源： 44個(gè)藍(lán)色LED，450 nm，測(cè)量光強(qiáng)度0.5 μmol m-2 s-1PAR，最大光化光強(qiáng)度1900 μmol m-2 s-1PAR，飽和脈沖強(qiáng)度4000 μmol m-2 s-1PAR
? 吸光系數(shù)測(cè)量光源：16個(gè)紅光（660 nm）和16個(gè)近紅外（780 nm）LED，用于測(cè)量樣品PAR吸光系數(shù)。
? 成像面積：工作距離18.5cm，成像面積 10×13 cm；工作距離22.5cm，成像面積11×15cm。
? 光強(qiáng)異質(zhì)性：測(cè)量區(qū)域光強(qiáng)異質(zhì)性小于±7％。
? 測(cè)量參數(shù)：Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等。
應(yīng)用領(lǐng)域
? 光合作用研究：可以在完全相同的條件下同時(shí)對(duì)大量樣品進(jìn)行成像
? 植物病理學(xué)：病斑部位（包括肉眼不可見(jiàn)時(shí)）成像以及病斑擴(kuò)散的時(shí)空動(dòng)力學(xué)
? 植物脅迫生 理學(xué)：肉眼不可見(jiàn)脅迫損傷的早期檢測(cè)
? 遺傳育種：出苗后大規(guī)模快速篩選高光合/抗旱/抗熱/抗凍/抗病等植株
? 突變株篩選：快速篩選模式植物的光合突變株、抗逆突變株、產(chǎn)氫微藻突變株等
? 微藻毒理學(xué)：不同毒物濃度多個(gè)重復(fù)的樣品一次測(cè)完，軟件自動(dòng)計(jì)算抑制比率
? 分子生物學(xué)：宏觀水平上檢測(cè)樣品的綠色熒光蛋白（GFP）熒光
? 其它多種擴(kuò)展研究
成像參數(shù)
Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), ETR, Abs, NIR和Red等
突變株的快速篩選
MAXI-IMAGING-PAM特別適合對(duì)幼苗、愈傷組織、微藻等進(jìn)行突變株的快速篩選，適合于與光合突變株、抗逆（抗旱、抗鹽、抗病等）突變株、產(chǎn)油/氫突變株等的快速篩選。
國(guó)外利用MAXI-IMAGING-PAM篩選突變株的典型客戶(hù)如拜耳、BASF、孟山都、先正達(dá)等大型跨國(guó)農(nóng)業(yè)巨頭，以及各大農(nóng)業(yè)育種、植物分子生物學(xué)等科研單位，例如澳大利亞植物功能基因組中心（阿德雷德大學(xué)）、德國(guó)尤里希表型植物表型研究中心（Julich Plant Phenotyping Centre）等等。
國(guó)內(nèi)約一半的MAXI-IMAGING-PAM客戶(hù)在進(jìn)行突變株快速篩選工作，主要分布于中國(guó)科學(xué)院、中國(guó)農(nóng)科院和各大高校。
突變株篩選實(shí)例一：國(guó)內(nèi)某客戶(hù)篩選的擬南芥突變株
突變株篩選實(shí)例二：產(chǎn)油突變株的篩選。Ajjawi et al, 2010, Plant Physiol., 152: 529-540.
突變株篩選實(shí)例三：光合突變株的篩選。Armbruster et al., 2010, Plant Cell, 22: 3439-3460.
調(diào)制葉綠素?zé)晒獬上駥?shí)例
葉片成像異質(zhì)性 1）葡萄葉片
2）荷花葉片
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水果的成像 1）草莓的成像
2）獼猴桃的成像
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突變株篩選
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植物病理研究
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熒光成像與CO2氣體交換的同步測(cè)量
（GFS-3000/IM-MAXI）：MAXI-探頭與GFS-3000聯(lián)用，在10 cm x 13 cm的面積上同步測(cè)量氣體交換與熒光成像。
產(chǎn)地：德國(guó)WALZ
代表文獻(xiàn)
數(shù)據(jù)來(lái)源：光合作用文獻(xiàn)Endnote數(shù)據(jù)庫(kù)
原始數(shù)據(jù)來(lái)源：Google Scholar
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